Article
Pattern of cytokine and chemokine production by THP-1 derived macrophages in response to live or heat-killed Mycobacterium bovis bacillus Calmette-Guérin Moreau strain
Registro en:
VASCONCELOS, Periela da Silva Sousa; et al. Pattern of cytokine and chemokine production by THP-1 derived macrophages in response to live or heat-killed Mycobacterium bovis bacillus Calmette-Guérin Moreau strain. Mem Inst Oswaldo Cruz, v.110, n.6, p.809-813, Sept. 2015.
0074-0276
10.1590/0074-02760140420
1678-8060
Autor
Vasconcelos, Periela da Silva Sousa
Seguins, Wellington da Silva
Luz, Eduardo de Souza
Pinho, Rosa Teixeira de
Resumen
Tuberculosis has great public health impact with high rates of mortality and the only prophylactic measure for
it is the Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccine. The present study evaluated the release of
cytokines [interleukin (IL)-1, tumour necrosis factor and IL-6] and chemokines [macrophage inflammatory protein
(MIP)-1α and MIP-1β] by THP-1 derived macrophages infected with BCG vaccine obtained by growing mycobacteria
in Viscondessa de Moraes Institute medium medium (oral) or Sauton medium (intradermic) to compare the effects
of live and heat-killed (HK) mycobacteria. Because BCG has been reported to lose viability during the lyophilisation
process and during storage, we examined whether exposing BCG to different temperatures also triggers differences
in the expression of some important cytokines and chemokines of the immune response. Interestingly, we observed
that HK mycobacteria stimulated cytokine and chemokine production in a different pattern from that observed with
live mycobacteria.