Survival and conjugation of Bacillus thuringiensis in a soil microcosm

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorVilas-Bôas, Laurival A.
dc.creatorVilas-Bôas, Gislayne F.L.T.
dc.creatorSaridakis, Halha O.
dc.creatorLemos, Manoel Victor F.
dc.creatorLereclus, Didier
dc.creatorArantes, Olivia M.N.
dc.date2014-05-27T11:19:53Z
dc.date2016-10-25T18:16:19Z
dc.date2014-05-27T11:19:53Z
dc.date2016-10-25T18:16:19Z
dc.date2000-03-01
dc.date.accessioned2017-04-06T00:56:45Z
dc.date.available2017-04-06T00:56:45Z
dc.identifierFEMS Microbiology Ecology, v. 31, n. 3, p. 255-259, 2000.
dc.identifier0168-6496
dc.identifierhttp://hdl.handle.net/11449/66123
dc.identifierhttp://acervodigital.unesp.br/handle/11449/66123
dc.identifier10.1111/j.1574-6941.2000.tb00691.x
dc.identifierWOS:000086001100009
dc.identifier2-s2.0-0342470947
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0168649600000027
dc.identifierhttp://dx.doi.org/10.1111/j.1574-6941.2000.tb00691.x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/887752
dc.descriptionThe survival and conjugation ability of sporogenic and asporogenic Bacillus thuringiensis strains were investigated in broth, in non-amended sterile clay soil monoculture and in mixed soil culture. The 75 kb pHT73 plasmid carrying an erythromycin resistance determinant and a cry1Ac gene was transferred in mating broth and soil microcosm. Survival of strains was assessed in soil monoculture and in mixed soil culture for up to 20 days. Sporogenic strains rapidly formed viable spores which were maintained until the end of the experiment. The asporogenic strains were no longer recovered after 8 days of incubation. This study shows that the environmental impact of asporogenic B. thuringiensis strains is lower than that of sporogenic B. thuringiensis strains. Thus, the use of asporogenic strains may significantly reduce any potential risk (gene transfer, soil and plant contamination) due to the dissemination of B. thuringiensis-based biopesticides in the environment. Copyright (C) 2000 Federation of European Microbiological Societies.
dc.languageeng
dc.relationFEMS Microbiology Ecology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBacillus thuringiensis
dc.subjectConjugation
dc.subjectPlasmid
dc.subjectSoil microcosm
dc.subjectSporulation mutant
dc.subjectmicrocosm
dc.subjectsoil microorganism
dc.subjectsurvival
dc.subjectbacterium conjugation
dc.subjectecosystem
dc.subjectgene transfer
dc.subjectmating
dc.subjectmutant
dc.subjectnonhuman
dc.subjectplasmid
dc.subjectpriority journal
dc.subjectsoil
dc.subjectsporogenesis
dc.titleSurvival and conjugation of Bacillus thuringiensis in a soil microcosm
dc.typeOtro


Este ítem pertenece a la siguiente institución