Article
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
Registro en:
PACHECO, Rodrigo Goulart et al. Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis. World Journal of Gastroenterology, v. 18, n. 32, p. 4278-4287, Aug. 2012.
1007-9327
10.3748/wjg.v18.i32.4278
2219-2840
Autor
Pacheco, Rodrigo Goulart
Esposito, Christiano Costa
Müller, Lucas C. M.
Castelo-Branco, Morgana T. L.
Quintella, Leonardo Pereira
Chagas, Vera Lucia A.
Souza, Heitor Siffert P. de
Schanaider, Alberto
Resumen
Leonardo Pereira Quintella. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento. AIM: To investigate whether butyrate or glutamine enemas could diminish inflammation in experimental
diversion colitis. METHODS: Wistar specific pathogen-free rats were submitted to a Hartmann’s end colostomy and treated with enemas containing glutamine, butyrate, or saline. Enemas were administered twice a week in the excluded segment of the colon from 4 to 12 wk after the surgical procedure. Follow-up colonoscopy was performed every 4 wk for 12 wk. The effect of treatment was evaluated using video-endoscopic and histologic scores and measuring interleukin-1β, tumor necrosis factor-alpha, and transforming growth factor beta production in organ cultures by enzyme linked immunosorbent assay. RESULTS: Colonoscopies of the diverted segment showed mucosa with hyperemia, increased number of vessels, bleeding and mucus discharge. Treatment with either glutamine or butyrate induced significant reductions in both colonoscopic (P < 0.02) and histological scores (P < 0.01) and restored the densities of collagen fibers in tissue (P = 0.015; P = 0.001), the
number of goblet cells (P = 0.021; P = 0.029), and the rate of apoptosis within the epithelium (P = 0.043; P = 0.011) to normal values. The high levels of cytokines in colon explants from rats with diversion colitis significantly decreased to normal values after treatment with butyrate or glutamine. CONCLUSION: The improvement of experimental diversion colitis following glutamine or butyrate enemas highlights the importance of specific luminal nutrients in the homeostasis of the colonic mucosa and supports their utilization for the treatment of human diversion colitis.