Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts

dc.creatorZych, Jaiesa
dc.creatorSpangenberg, Lucia
dc.creatorStimamiglio, Marco Augusto
dc.creatorAbud, Ana Paula Ressetti
dc.creatorShigunov, Patrícia
dc.creatorMarchini, Fabrício Klerynton
dc.creatorKuligovski, Crisciele
dc.creatorCofré, Axel Helmut Rulf
dc.creatorSchittini, Andressa V.
dc.creatorAguiar, Alessandra Melo de
dc.creatorSenegaglia, Alexandra Cristina
dc.creatorBrofman, Paulo Roberto Slud
dc.creatorGoldenberg, Samuel
dc.creatorDallagiovanna, Bruno
dc.creatorNaya, Hugo
dc.creatorDominguez, Alejandro Correa
dc.date2014-12-12T00:08:23Z
dc.date2015-11-15T06:30:04Z
dc.date2014
dc.date.accessioned2023-09-26T22:16:14Z
dc.date.available2023-09-26T22:16:14Z
dc.identifierZYCH, Jaiesa et al. Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts. Stem Cells and Development, v. 23, n. 22, p. 2791-2802, 2014.
dc.identifier1547-3287
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9158
dc.identifier10.1089/scd.2013.0496
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877105
dc.descriptionO artigo encontra-se disponível em acesso aberto no site do Editor.
dc.descriptionThis work was supported by grants from Ministério da Saúde and Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, FIOCRUZ-Pasteur Research Program, and Fundação Araucária. L.S. received fellowship from ANII (Agencia Nacional de Investigación e Innovación, Uruguay); S.G., J.Z., and B.D. from CNPq; P.S. from FIOCRUZ; and A.C. from Fundação Araucária.
dc.descriptionAlthough fibroblasts and multipotent stromal/stem cells, including adipose-derived stromal cells (ADSCs), have been extensively studied, they cannot be clearly distinguished from each other. We, therefore, investigated the cellular and molecular characteristics of ADSCs and fibroblasts. ADSCs and fibroblasts share several morphological similarities and surface markers, but were clearly found to be different types of cells. Contrary to previous reports, fibroblasts were not able to differentiate into adipocytes, osteoblasts, or chondrocytes. Polysome-bound mRNA profiling revealed that*1,547 genes were differentially expressed (DE) in the two cell types; the genes were related to cell adhesion, the extracellular matrix, differentiation, and proliferation. These findings were confirmed by functional analyses showing that ADSCs had a greater adhesion capacity than fibroblasts; the proliferation rate of fibroblasts was also higher than that of ADSCs. Importantly, 185 DE genes were integral to the plasma membrane and, thus, candidate markers for ADSC isolation and manipulation. We also observed that an established marker of fibroblasts and ADSCs, CD105, was overexpressed in ADSCs at both mRNA and protein levels. CD105 expression seemed to be related to differentiation capacity, at least for adipogenesis. This study shows that ADSCs and fibroblasts are distinct cell types. These findings should be taken into account when using these two cell types in basic and therapeutic studies.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMary Ann Liebert, Inc
dc.rightsopen access
dc.subjectFibroblasts
dc.subjectStromal Cells
dc.subjectCélulas del Estroma
dc.subjectFibroblastos
dc.subjectCélulas Estromais
dc.titlePolysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts
dc.typeArticle


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