Article
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126
Registro en:
SANTOS, Edvan do Carmo et. al. A description of the hemolytic component in sickle leg ulcer: The role of circulating miR-199a-5p, miR-144, and miR-126. Biomolecules, v. 12, n. 2, p. 1-10, 2022.
2218-273X
10.3390/biom12020317
Autor
Santos, Edvan do Carmo
Melo, Gabriela Imbassahy Valentim
Santana, Paulo Vinícius Bispo
Quadros, Idaiara Graziele Silva
Yahouédéhou, Sètondji Cocou Modeste Alexandre
Guarda, Caroline Conceição da
Santiago, Rayra Pereira
Fiuza, Luciana Magalhães
Carvalho, Suéllen Pinheiro
Adorno, Elisângela Vitória
Kaneto, Carla Martins
Fonseca, Teresa Cristina Cardoso
Goncalves, Marilda Souza
Aleluia, Milena Magalhães
Resumen
Universidade Estadual de Santa Cruz (UESC) Sickle leg ulcers (SLU) are malleoli lesions with exuberant hemolytic pathophysiology. The microRNAs are potential genetic biomarkers for several pathologies. Thereby, we aimed to assess the expression of circulating miR-199a-5p, miR-144, and miR-126 in association with hemolytic biomarkers in SLU. This cross-sectional study included 69 patients with sickle cell disease, 52 patients without SLU (SLU-) and 17 patients with active SLU or previous history (SLU+). The results demonstrated elevated expression of circulating miR-199a-5p and miR-144 in SLU+ patients while miR-126 expression was reduced. Circulating miR-199a-5p and miR-144 were associated with hemolytic biomarkers such as LDH, indirect bilirubin, AST, GGT, iron, ferritin, RBC, hemoglobin, and NOm, in addition to association with impaired clinical profile of SLU. Furthermore, in silico analyses indicated interactions of miR-199a-5p with HIF1A, Ets-1, and TGFB2 genes, which are associated with vasculopathy and reduced NO. In contrast, miR-126 was associated with an attenuating clinical profile of SLU, in addition to not characterizing hemolysis. In summary, this study demonstrates, for the first time, that hemolytic mechanism in SLU can be characterized by circulating miR-199a-5p and miR-144. The circulating miR-126 may play a protective role in SLU. Thus, these microRNAs can support to establish prognosis and therapeutic strategy in SLU.