Article
Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)
Registro en:
BANDEIRA, R. D. C. C. et al. Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS). Química Nova, São Paulo, v. 35, n. 1, p. 66-71, 2012.
0100-4042
Autor
Bandeira, Raquel Duarte da Costa Cunha
Uekane, Thais Matsue
Cunha, Carolina Passos da
Geaquinho, Luths Raquel de Oliveira
Cunha, Valnei Smarçaro da
Caixeiro, Janaína Marques Rodrigues
Godoy, Ronoel Luiz de Oliveira
Cruz, Marcus Henrique Campino de La
Resumen
A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.