Article
Development of nuclear DNA probes for the typing of Trypanososma cruzi
Registro en:
TAKEUCHI, A. Mayumi; TRAUB-CSEKO, Yara M. Development of nuclear DNA probes for the typing of Trypanososma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 3, p. 307-315, July/Sept. 1991.
0074-0276
10.1590/S0074-02761991000300005
1678-8060
Autor
Takeuchi, A. Mayumi
Traub-Cseko, Yara M.
Resumen
We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level.