Toxocariasis: Serological diagnosis by indirect antibody competition elisa

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorNunes, Caris Maroni
dc.creatorTundisi, Regina Nardini
dc.creatorHeinemann, Marcos Bryan
dc.creatorOgassawara, Saemi
dc.creatorRichtzenhain, Leonardo José
dc.date2014-05-27T11:19:49Z
dc.date2016-10-25T18:16:02Z
dc.date2014-05-27T11:19:49Z
dc.date2016-10-25T18:16:02Z
dc.date1999-12-01
dc.date.accessioned2017-04-06T00:55:32Z
dc.date.available2017-04-06T00:55:32Z
dc.identifierRevista do Instituto de Medicina Tropical de Sao Paulo, v. 41, n. 2, p. 95-100, 1999.
dc.identifier0036-4665
dc.identifierhttp://hdl.handle.net/11449/65970
dc.identifierhttp://acervodigital.unesp.br/handle/11449/65970
dc.identifier10.1590/S0036-46651999000200006
dc.identifierS0036-46651999000200006
dc.identifier2-s2.0-0033091502
dc.identifierhttp://dx.doi.org/10.1590/S0036-46651999000200006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/887614
dc.descriptionToxocariasis is caused by infection of man by Toxocara canis and Toxocara, cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody. in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%).
dc.languageeng
dc.relationRevista do Instituto de Medicina Tropical de São Paulo
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectImmunodiagnosis
dc.subjectIndirect antibody competition ELISA
dc.subjectToxocara canis
dc.subjectToxocariasis
dc.subjectVisceral larva migrans
dc.subjecthelminth antibody
dc.subjectimmunoglobulin G
dc.subjectparasite antigen
dc.subjectanimal
dc.subjectblood
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjecthuman
dc.subjectimmunology
dc.subjectmethodology
dc.subjectmouse
dc.subjectsensitivity and specificity
dc.subjectserology
dc.subjecttoxocariasis
dc.subjectAnimals
dc.subjectAntibodies, Helminth
dc.subjectAntigens, Helminth
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunoglobulin G
dc.subjectMice
dc.subjectSensitivity and Specificity
dc.subjectSerologic Tests
dc.titleToxocariasis: Serological diagnosis by indirect antibody competition elisa
dc.typeOtro


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