A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.

Barral Netto, Manoel; Barral, Aldina Maria Prado

Article

Registro en:

BARRAL-NETTO, M.; BARRAL, A. A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding. Memórias do Instituto Oswaldo Cruz, v. 81, n. 3, p. 343-345, 1986.

0074-0276

https://www.arca.fiocruz.br/handle/icict/7467

https://repositorioslatinoamericanos.uchile.cl/handle/2250/8874947

Autor

Barral Netto, Manoel

Barral, Aldina Maria Prado

Institución

Instituto de Comunicação e Informação Científica e Tecnológica em Saúde (Brasil)

Resumen

We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.

Materias

Concanavalin A

Mononuclear cells

Visceral leishmaniasis

Concanavalina A/metabolismo

Leishmaniose Visceral/sangue

Monócitos/metabolismo

Técnicas Imunoenzimáticas

Humanos

Ligação Proteica

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