Article
Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
Registro en:
CONZENTINO, Marcelo S. et al. Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads. Analytical Biochemistry, v.631, n. 114360, p. 1-6, 2021.
0003-2697
10.1016/j.ab.2021.114360
Autor
Conzentino, Marcelo S.
Santos, Tatielle P.C.
Selim, Khaled A.
Wagner, Berenike
Alford, Janette T.
Deobald, Nelli
Paula, Nigela M.
Rego, Fabiane G. M.
Zanette, Dalila Lucíola
Aoki, Mateus Nóbrega
Nardin, Jeanine M.
Huergo, Maria C. C.
Reis, Rodrigo A.
Huergo, Luciano F.
Resumen
To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative
analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.