HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland

dc.creatorDamasceno, Karine Araújo
dc.creatorFerreira, Enio
dc.creatorLima, Alessandra Estrela
dc.creatorGamba, Conrado de Oliveira
dc.creatorMiranda, Fernanda Freitas
dc.creatorAlves, Mariana Rezende
dc.creatorRocha, Rafael Malagoli
dc.creatorBarros, André Luis Branco de
dc.creatorCassali, Geovanni Dantas
dc.date2017-02-08T17:54:20Z
dc.date2017-02-08T17:54:20Z
dc.date2016
dc.date.accessioned2023-09-26T21:46:23Z
dc.date.available2023-09-26T21:46:23Z
dc.identifierDAMASCENO, K. A. et al. HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland. PLoS ONE, v. 11, n. 8, p. e0160419, 2016.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/17751
dc.identifier10.1371/journal.pone.0160419
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873542
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Conselho Nacional de Desenvolvimento e Pesquisa (CNPq) and Fundação de amparo a pesquisa do Estado de Minas Gerais (FAPEMIG).
dc.descriptionVersican expression promotes tumor growth by destabilizing focal cell contacts, thus impeding cell adhesion and facilitating cell migration. It not only presents or recruits molecules to the cell surface, but also modulates gene expression levels and coordinates complex signal pathways. Previously, we suggested that the interaction between versican and human epidermal growth factor receptors may be directly associated with tumor aggressiveness. Thus, the expression of EGFR and HER-2 in these neoplasms may contribute to a better understanding of the progression mechanisms in malignant mammary tumors. The purpose of this study was to correlate the gene and protein expressions of EGFR and HER2 by RNA In Situ Hybridization (ISH) and immunohistochemistry (IHC), respectively, and their relationship with the versican expression in carcinomas in mixed tumors and carcinosarcomas of the canine mammary gland. The results revealed that EGFR mRNA expression showed a significant difference between in situ and invasive carcinomatous areas in low and high versican expression groups. Identical results were observed in HER-2 mRNA expression. In immunohistochemistry analysis, neoplasms with low versican expression showed greater EGFR immunostaining in the in situ areas than in invasive areas, even as the group presenting high versican expression displayed greater EGFR and HER-2 staining in in situ areas. Significant EGFR and HER-2 mRNA and protein expressions in in situ carcinomatous sites relative to invasive areas suggest that these molecules play a role during the early stages of tumor progression.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectGlandulas mamarias de animais
dc.subjectGenes EGFR
dc.subjectCarcinoma
dc.subjectProteínas
dc.subjectTumores mamarios animais
dc.subjectAnimais
dc.subjectAnimal mammary glands
dc.subjectGene EGFR
dc.subjectCarcinoma
dc.subjectProteins
dc.subjectAnimal mammary tumors
dc.subjectAnimal
dc.titleHER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
dc.typeArticle


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