Article
Human heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves
Registro en:
LEITOLIS, Amanda et al. Human heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves. Int. J. Mol. Sci. , v. 20, n. 1279, p. 1-18, 2019.
1422-0067
10.3390/ijms20061279
1422-0067
Autor
Leitolis, Amanda
Suss, Paula Hansen
Roderjan, João Gabriel
Angulski, Addeli Bez Batti
Costa, Francisco Diniz Affonso da
Stimamiglio, Marco Augusto
Dominguez, Alejandro Correa
Resumen
Extracellular vesicles (EVs) are particles released from different cell types and represent key components of paracrine secretion. Accumulating evidence supports the beneficial effects of EVs for tissue regeneration. In this study, discarded human heart tissues were used to isolate human heart-derived extracellular vesicles (hH-EVs). We used nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) to physically characterize hH-EVs and mass spectrometry (MS) to profile the protein content in these particles. The MS analysis identified a total of 1248 proteins. Gene ontology (GO) enrichment analysis in hH-EVs revealed the proteins involved in processes, such as the regulation of cell death and response to wounding. The potential of hH-EVs to induce proliferation, adhesion, angiogenesis and wound healing was investigated in vitro. Our findings demonstrate that hH-EVs have the potential to induce proliferation and angiogenesis in endothelial cells, improve wound healing and reduce mesenchymal stem-cell adhesion. Last, we showed that hH-EVs were able to significantly promote mesenchymal stem-cell recellularization of decellularized porcine heart valve leaflets. Altogether our data confirmed that hH-EVs modulate cellular processes, shedding light on the potential of these particles for tissue regeneration and for scaffold recellularization.