Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction

dc.creatorSilva, Tatiana Araújo
dc.creatorFerreira, Luis Felipe de Carvalho
dc.creatorPereira, Mirian Claudia de Souza
dc.creatorCalvet, Claudia Magalhães
dc.date2020-05-04T19:03:50Z
dc.date2020-05-04T19:03:50Z
dc.date2019
dc.date.accessioned2023-09-26T21:23:01Z
dc.date.available2023-09-26T21:23:01Z
dc.identifierSILVA, Tatiana Araújo et al. Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction. International Journal of Molecular Sciences, v. 20, n. 4836, p. 1-21, 2019.
dc.identifier1661-6596
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/41093
dc.identifier10.3390/ijms20194836
dc.identifier1422-0067
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872213
dc.descriptionTransforming growth factor beta (TGF-β) is a determinant for inflammation and fibrosis in cardiac and skeletal muscle in Chagas disease. To determine its regulatory mechanisms, we investigated the response of Trypanosoma cruzi-infected cardiomyocytes (CM), cardiac fibroblasts (CF), and L6E9 skeletal myoblasts to TGF-β. Cultures of CM, CF, and L6E9 were infected with T. cruzi (Y strain) and treated with TGF-β (1-10 ng/mL, 1 h or 48 h). Fibronectin (FN) distribution was analyzed by immunofluorescence and Western blot (WB). Phosphorylated SMAD2 (PS2), phospho-p38 (p-p38), and phospho-c-Jun (p-c-Jun) signaling were evaluated by WB. CF and L6E9 showed an increase in FN from 1 ng/mL of TGF-β, while CM displayed FN modulation only after 10 ng/mL treatment. CF and L6E9 showed higher PS2 levels than CM, while p38 was less stimulated in CF than CM and L6E9. T. cruzi infection resulted in localized FN disorganization in CF and L6E9. T. cruzi induced an increase in FN in CF cultures, mainly in uninfected cells. Infected CF cultures treated with TGF-β showed a reduction in PS2 and an increase in p-p38 and p-c-Jun levels. Our data suggest that p38 and c-Jun pathways may be participating in the fibrosis regulatory process mediated by TGF-β after T. cruzi infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectTGF-β
dc.subjectFibrose cardíaca
dc.subjectMatriz extracelular
dc.subjectCaminhos de sinalização
dc.subjectTrypanosoma cruzi
dc.subjectTGF-β
dc.subjectHeart fibrosis
dc.subjectExtracellular matrix
dc.subjectSignaling pathways
dc.subjectSMAD2
dc.subjectp38 MAPK
dc.subjectc-Jun
dc.titleDifferential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
dc.typeArticle


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