Otro
Frequent p53 mutations and occasional loss of chromosome 4 in invasive bladder carcinoma induced by N-butyl-N-(4-hydroxybutil)nitrosamine in B6D2F1 mice
Registro en:
Molecular Carcinogenesis, v. 21, n. 1, p. 70-79, 1998.
0899-1987
10.1002/(SICI)1098-2744(199801)21:1<70::AID-MC9>3.0.CO;2-T
2-s2.0-0031885711
Autor
Ogawa, Kumiko
Uzvolgyi, Éva
St John, Margaret K.
Oliveira, Maria Luiza de
Arnold, Lora
Cohen, Samuel Monroe
Resumen
B6D2F1 mice (45/group) were treated with N-butyl-N-(4- hydroxybutyl)nitrosamine (BBN) or uracil as follows: Group 1 received 0.05% BBN in drinking water for the entire experiment, Group 2 received 5 mg of BBN by gastric gavage in 0.1 mL of 20% ethanol twice per week for 10 wk, Group 3 received a 2.5% uracil-containing diet for the entire experiment, and Group 4 was controls (received 0.1 mL of 20% ethanol by gavage twice per week for 10 wk). The surviving mice in Group 1 were killed after week 26 and those in the other groups after week 30. By week 15, three of 11 Group 1 and one of 15 Group 2 mice had bladder carcinoma. By 26 and 30 wk, respectively, invasive carcinomas were observed in 33 of 34 and six of 21 mice in Groups 1 and 2 and renal pelvic carcinomas in 11 of 34 and three of 21 mice in Groups 1 and 2. Four of 19 uracil-treated mice had bladder nodular hyperplasia. By polymerase chain reaction-single-strand conformation polymorphism and sequence analyses, 16 of 20 and two of five bladder carcinomas from Groups 1 and 2, respectively, showed mutations in the p53 gene. Ha-ras mutation was present in one case. Loss of heterozygosity analysis with simple-sequence length polymorphism markers for chromosome 4 showed that 10 of 21, two of 15, and nine of 13 mice in Groups 1-3, respectively, had heterozygous or homozygous deletions. B6D2F1 mice are therefore susceptible to the urothelial carcinogenic effects of BBN and develop frequent p53 mutations and chromosome 4 deletions. Chromosome 4 deletions were also seen with uracil.
Materias
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Low RKIP expression associates with poor prognosis in bladder cancer patients
Afonso, Julieta; Longatto-Filho, Adhemar; Martinho, Olga; Lobo, Francisco; Amaro, Teresina; Reis, Rui M.; Santos, Lúcio L. -
Urinary bladder washings in autopsies. A cytopathologic study of 63 cases
Tani, E. M.; Montenegro, M. R.; Vana de Camargo, J. L. -
Effects of uracil calculi on cell growth and apoptosis in the BBN- initiated Wistar rat urinary bladder mucosa
Oliveira, Susie Vieira de; Camargo, João Lauro Viana de; Cardoso, Paulo Roberto; Padovani, Carlos Roberto; Fukushima, Shoji