Article
Leishmania braziliensis Infection Enhances Toll-Like Receptors 2 and 4 Expression and Triggers TNF-α and IL-10 Production in Human Cutaneous Leishmaniasis
Registro en:
POLARI, Ludmila Porto Mendes et al. Leishmania braziliensis Infection Enhances Toll-Like Receptors 2 and 4 Expression and Triggers TNF-α and IL-10 Production in Human Cutaneous Leishmaniasis. Frontiers in Cellular and Infection Microbiology, v. 9, n. 120, p. 1-11, May 2019.
2235-2988
10.3389/fcimb.2019.00120
Autor
Polari, Ludmila Porto Mendes
Carneiro, Pedro Paulo Oliveira
Macedo, Michael Nascimento
Machado, Paulo Roberto Lima
Scott, Phillip
Carvalho Filho, Edgar Marcelino
Bacellar, Maria Olívia Amado Ramos
Resumen
National Institute of Health
(NIH) grant AI136032. The funders had no role in study design,
data collection and analysis, decision to publish, or preparation
of the manuscript. Cutaneous leishmaniasis (CL) caused by infection with Leishmania braziliensis is
characterized by an exaggerated inflammatory response that controls the parasite
burden, but also contributes to pathology. While myeloid cells are required to eliminate
the parasite, recent studies indicate that they may also participate in the inflammatory
response driving disease progression. The innate immune response to leishmania is
driven in part by the Toll-like receptors (TLRs) TLR2, TLR4, and TLR9. In this study,
we used flow cytometric analysis to compare TLR2 and TLR4 expression in monocyte
subsets (classical, intermediate, and non-classical) fromCL patients and healthy subjects
(HS). We also determined if there was an association of either the pro-inflammatory
cytokine TNF or the anti-inflammatory cytokine IL-10 with TLR2 or TLR4 expression levels
after L. braziliensis infection. In vitro infection with L. braziliensis caused CL monocytes
to up-regulate TLR2 and TLR4 expression. We also found that intermediate monocytes
expressed the highest levels of TLR2 and TLR4 and that infected monocytes produced
more TNF and IL-10 than uninfected monocytes. Finally, while classical and intermediate
monocytes were mainly responsible for TNF production, classical monocytes were
the main source of IL-10. Collectively, our studies revealed that up-regulated TLR2/4
expression and TNF production by intermediate/inflammatory subsets of monocytes
from patients correlates with detrimental outcome of cutaneous leishmaniasis.
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Rol del receptor de lipoproteínas de alta densidad, SR-BI, en el cierre del tubo neural: evidencias a partir de un ratón deficente en SR-BI y estudio de la participación de este receptor en la anencefalia humana
Busso-Perkins, Dolores; PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE (2017)El receptor Scavenger Class B type I (SR-BI) une lipoproteínas de alta densidad (HDL) y media el transporte bidireccional de lípidos entre la lipoproteína y la célula, incluyendo colesterol y vitamina E. Alrededor de la ...