Article
Kinetics of mesenchymal and hematopoietic stem cells mobilization by G-CSF and its impact on the cytokine microenvironment in primary cultures
Registro en:
GARCIA, Nadja Pinto et al. Kinetics of mesenchymal and hematopoietic stem cells mobilization by G-CSF and its impact on the cytokine microenvironment in primary cultures. Cellular Immunology, vol. 293, n. 2015, p.1–9, 2014
0008-8749
10.1016/j.cellimm.2014.09.006
Autor
Garcia, Nadja Pinto
Leon, Elisa Brosina de
Costa, Allyson Guimarães da
Tarragô, Andréa Monteiro
Pimentel, João Paulo
Fraporti, Liziara
Araujo, Fernanda Fortes de
Campos, Fernanda Magalhães Freire
Carvalho, Andréa Teixeira
Martins Filho, Olindo Assis
Malheiro, Adriana
Resumen
In this study, we demonstrate that G-CSF administration triggers distinct kinetics of stem cell-SC mobilization with early raise of hematopoietic-HSC and late increase of mesenchymal-MSC in bone marrow-BM and peripheral blood-PB. The cytokine microenvironment observed following primary cultures showed an overall G-CSF dose-dependent profile with a clear mixed pro-inflammatory/regulatory pattern. Moreover, primary cultures performed at the peak of MSC/HSC ratio, showed distinct cytokine patterns, with higher IL-10, TNF-a and IL-17A observed for BM and enhanced IL-10, IL-2 and IFN-c for PB harvested cells. Positive correlation was observed between BM-MSC and the levels of TNF-a, IL-10 and IL-17A whereas negative correlation was found between IL-10 and BM-HSC. An opposite association was observed between IL-10 and PB-HSC. Our results support the hypothesis that MSC and HSC harvested from BM and PB display differential functional properties that should be considered when electing the SC sources available for cell therapy applied in clinical protocols.