Article
Improvement of direct agglutination test (DAT) for laboratory diagnosis of visceral leishmaniasis in Brazil
Registro en:
OLIVEIRA, Edward José de et al. Improvement of direct agglutination test (DAT) for laboratory diagnosis of visceral leishmaniasis in Brazil. Trans R Soc Trop Med Hyg., v. 103, n. 12, p. 1279-1281, 2009. doi: 10.1016/j.trstmh.2009.04.007
0035-9203
10.1016/j.trstmh.2009.04.007
Autor
Oliveira, Edward José de
Pedras, Mariana Junqueira
Assis, Isabella Elias Morais de
Rabello, Ana Lucia Teles
Resumen
We previously standardized the direct agglutination test (DAT) to detect antiLeishmania chagasi promastigote antibodies (DAT-LPC) with good sensitivity and specificity for diagnosing visceral. leishmaniasis (VL). In this paper, we present a technical upgrade by introducing some modifications into the antigen preparation. This antigen was evaluated in DAT (DAT-Mod) using 61 sera samples from VL patients and 96 samples from patients with other diseases. The DAT-Mod presented a cut-off of 1:100, satisfactory reproducibility (VC <5.8), sensitivity of 93.4%, specificity of 96.9%, and diagnostic efficiency of 95.5%. The improvement in antigen preparation reduced inter-batch variations and resulted in a high test performance. (C) 2009 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved 2099-12-31