Article
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
Registro en:
RODRIGUES, C. A. T. et al. IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. Veterinary Immunology and Immunopathology, v. 127, n. 3-4, p. 382-388, 2009.
0165-2427
10.1016/j.vetimm.2008.10.324
Autor
Rodrigues, Cleusa Alves Theodoro
Batista, Luís Fábio da Silva
Teixeira Filho, Roberto Santos
Santos, Claire da Silva
Pinheiro, Cristiane Garboggini Melo de
Almeida, Taís Fontoura de
Freitas, Luiz Antonio Rodrigues de
Resumen
While the response to Leishmania spp. is well characterized in mice and humans, much less
is known concerning the canine immune response, particularly soon after exposure to the
parasite. Early events are considered to be a determinant of infection outcome. To
investigate the dog’s early immune response to L. chagasi, an in vitro priming system (PIV)
using dog naı¨ve PBMC was established. Until now, dog PIV immune response to L. chagasi
has not been assessed. We co-cultivated PBMC primarily stimulated with L. chagasi in vitro
with autologous infected macrophages and found that IFN-g mRNA is up-regulated in
these cells compared to control unstimulated cells. IL-4 and IL-10 mRNA expression by L.
chagasi-stimulated PBMC was similar to control unstimulated PBMCwhen incubated with
infected macrophages. Surprisingly, correlation studies showed that a lower IFN-g/IL-4
expression ratio correlated with a lower percentage of infection. We propose that the
direct correlation between IFN-g/IL-4 ratio and parasite load is dependent on the higher
correlation of both IFN-g and IL-4 expression with lower parasite infection. This PIV
system was shown to be useful in evaluating the dog immune response to L. chagasi, and
results indicate that a balance between IFN-g and IL-4 is associated with control of
parasite infection in vitro.