Article
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells
Registro en:
OLIVEIRA, Iara dos Santos da Silva et al. Vernonia polysphaera Baker: Antiinflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells. PLoS ONE, v. 14, n. 12, p. 1-19, 12 Dec. 2019.
1932-6203
10.1371/journal.pone.0225275
Autor
Oliveira, Iara dos Santos da Silva
Colares, Aracélio Viana
Cardoso, Flávia de Oliveira
Tellis, Carla Junqueira Moragas
Chagas, Maria do Socorro dos Santos
Behrens, Maria Dutra
Calabrese, Kátia da Silva
Souza, Fernando Almeida
Silva, Ana Lúcia Abreu
Resumen
Species of the Vernonia genius are widely distributed across the world. In traditional communities, they are commonly used in popular medicine for the treatment of inflammatory diseases. The objective of the present study was to evaluate the anti-inflammatory activity of
Vernonia polysphaera Baker hydroalcoholic extract. A λ-carrageenan-induced paw edema
and peritonitis model was established in BALB/c mice. The in vitro activity of the extract was
measured on LPS-stimulated RAW 264.7 cells. There was no toxic effect on mice or on the
cells treated with the extract. Animals treated with V. polysphaera extract demonstrated inhibition of paw edema in comparison with the untreated animals at all the analyzed doses. In
peritonitis, treatment with the extract at a dose of 500 mg/kg resulted in a lower total leukocyte count in the peritoneal fluid and blood and lower levels of IL-1β, IL-6, TNF-α and PGE-2
than the control group. Cells treated with 50 and 100 μg/mL of the extract exhibited lower
levels of nitrite and pro-inflammatory cytokine production and lower COX-2, NF-κB expression. The V. polysphaera extract demonstrated an anti-inflammatory effect, interfering with
cell migration, reducing pro-inflammatory cytokine levels and COX-2 expression and consequent interference with PGE-2, as well as inhibiting NF-κB transcription.