Article
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene
Registro en:
FONSECA, Érica Lourenço da; VICENTE, Ana Carolina Paulo. Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene. Antimicrobial Agents and Chemotherapy, v. 56, n. 6, p. 3392–3394, Jun. 2012.
0066-4804
Autor
Fonseca, Érica Lourenço da
Vicente, Ana Carolina Paulo
Resumen
Integrons are natural expression vectors due to the presence of an intrinsic promoter (Pc). Although rare, gene cassettes can harbor their own promoter. This study determined the functionality of an internal promoter in the qnrVC1 cassette whose presence
was suggested by a level of transcription similar to that of the preceding cassette (aadA2) and confirmed by in silico analysis. Its functionality was determined by 5= rapid amplification of cDNA ends (RACE) and cloning into promoter-probe vectors. PqnrVC
was found in the qnrVC cassette family, stressing its role in contributing to resistance manifestation.