dc.creatorManso, Pedro Paulo de Abreu
dc.creatorBrito-Gitirana, Lycia de
dc.creatorMachado, Marcelo Pelajo
dc.date2018-08-27T11:33:48Z
dc.date2018-08-27T11:33:48Z
dc.date2009
dc.date.accessioned2023-09-26T21:05:51Z
dc.date.available2023-09-26T21:05:51Z
dc.identifierMANSO, Pedro Paulo de Abreu; BRITO-GITIRANA, Lycia de; MACHADO, Marcelo Pelajo. Localization of hematopoietic cells in the bullfrog (Lithobates catesbeianus). Cell Tissue Res., v.337, p.301-312, 2009.
dc.identifier0302-766X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28363
dc.identifier10.1007/s00441-009-0803-0
dc.identifier1432-0878
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8869092
dc.descriptionAmphibians represent the first phylogenetic group to possess hematopoietic bone marrow. However, adult amphibian hematopoiesis has only been described in a few species and with conflicting data. Bone marrow, kidney, spleen, liver, gut, stomach, lung, tegument, and heart were therefore collected from adult Lithobates catesbeianus and investigated by light microscopy and immunohistochemical methods under confocal laser microscopy. Our study demonstrated active hematopoiesis in the bone marrow of vertebrae, femur, and fingers and in the kidney, but no hematopoietic activity inside other organs including the spleen and liver. Blood cells were identified as a heterogeneous cell population constituted by heterophils, basophils, eosinophils, monocytes, erythrocytic cells, lymphocytes, and their precursors. Cellular islets of the thrombocytic lineage occurred near sinusoids of the bone marrow. Antibodies against CD34, CD117, stem cell antigen, erythropoietin receptor, and the receptor for granulocyte colony-stimulating factor identified some cell populations, and some circulating immature cells were seen in the bloodstream. Thus, on the basis of these phylogenetic features, we propose that L. catesbeianus can be used as an important model for hematopoietic studies, since this anuran exhibits hematopoiesis characteristics both of lower vertebrates (renal hematopoiesis) and of higher vertebrates (bone marrow hematopoiesis).
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag (Germany)
dc.rightsrestricted access
dc.subjectHematopoese
dc.subjectHistoquímica
dc.subjectImunohistoquímica
dc.subjectAnfíbios
dc.subjectLithobates catesbeianus [Anura]
dc.subjectHematopoiesis
dc.subjectHistochemistry
dc.subjectImunohistochemistry
dc.subjectAmphibian
dc.subjectLithobates catesbeianus [Anura]
dc.titleLocalization of hematopoietic cells in the bullfrog (Lithobates catesbeianus)
dc.typeArticle


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