IgG, IgM and IgA antibody response for the diagnosis and follow-up of paracoccidioidomycosis: Comparison of counterimmunoelectrophoresis and complement fixation

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBueno, J. P.
dc.creatorMendes-Giannini, Maria José Soares
dc.creatorDel Negro, G. M. B.
dc.creatorAssis, C. M.
dc.creatorTakiguti, C. K.
dc.creatorShikanai-Yasuda, M. A.
dc.date2014-05-27T11:18:15Z
dc.date2016-10-25T18:14:32Z
dc.date2014-05-27T11:18:15Z
dc.date2016-10-25T18:14:32Z
dc.date1997-07-22
dc.date.accessioned2017-04-06T00:49:54Z
dc.date.available2017-04-06T00:49:54Z
dc.identifierJournal of Medical and Veterinary Mycology, v. 35, n. 3, p. 213-217, 1997.
dc.identifier0268-1218
dc.identifier1369-3786
dc.identifier1460-2709
dc.identifierhttp://hdl.handle.net/11449/65151
dc.identifierhttp://acervodigital.unesp.br/handle/11449/65151
dc.identifier10.1080/02681219780001161
dc.identifier2-s2.0-0030836629
dc.identifier2-s2.0-79961230907
dc.identifier0000-0002-8059-0826
dc.identifierhttp://dx.doi.org/10.1080/02681219780001161
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/886901
dc.descriptionIgG, IgM and IgA antibodies to GP43 (glycoprotein fraction of Paracoccidioides brasiliensis) were measured by ELISA in 63 samples from 23 patients with paracoccidioidomycosis before and twice after chemotherapy was started. Antibodies against P. brasiliensis were detected by indirect immunofluorescence (IF) (IgG, IgM and IgA isotypes), counterimmunoelectrophoresis (CIE) and complement fixation. Two control groups composed of 19 healthy individuals and 12 patients with other diseases (six with histoplasmosis, three with tuberculosis and three with other mycoses). The highest efficiency percentages were found with IgG and IgA- ELISA (100%), IgG-IF (96.2%), CIE (94.4%) and the lowest with CF (75.9%). Highest positive and negative predictive values (100%) were observed for IgG and IgA ELISA. IgG and IgM-ELISA antibodies are more often found in patients with acute than chronic disease (P = 0.01). Four to six months after treatment follow-up showed decreased levels of IgG and IgM-ELISA for acute cases and decreased titres of CIE for chronic cases in relation to pretreatment levels. This study suggests that IgG-ELISA anti-GP43 represents a good marker to monitor clinical response to therapy.
dc.languageeng
dc.relationJournal of Medical and Veterinary Mycology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDiagnosis
dc.subjectELISA anti-GP43
dc.subjectP. brasiliensis
dc.subjectParacoccidioidomycosis
dc.subjectglycoprotein
dc.subjectimmunoglobulin a antibody
dc.subjectimmunoglobulin g antibody
dc.subjectimmunoglobulin m antibody
dc.subjectantibody response
dc.subjectbiopsy
dc.subjectcomplement fixation
dc.subjectcontrolled study
dc.subjectcounter immunoelectrophoresis
dc.subjectenzyme linked immunosorbent assay
dc.subjectfollow up
dc.subjectfungus identification
dc.subjecthistoplasma capsulatum
dc.subjecthuman
dc.subjectimmunofluorescence
dc.subjectmajor clinical study
dc.subjectparacoccidioides brasiliensis
dc.subjectsouth american blastomycosis
dc.subjectsputum analysis
dc.subjectAcute Disease
dc.subjectAntibodies, Fungal
dc.subjectAntibody Formation
dc.subjectAntifungal Agents
dc.subjectChronic Disease
dc.subjectComplement Fixation Tests
dc.subjectCounterimmunoelectrophoresis
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectHumans
dc.subjectImmunoglobulin A
dc.subjectImmunoglobulin G
dc.subjectImmunoglobulin M
dc.subjectReference Values
dc.subjectAjellomyces capsulatus
dc.subjectFungi
dc.subjectParacoccidioides brasiliensis
dc.subjectparacoccidioidomycosis brasiliensis
dc.titleIgG, IgM and IgA antibody response for the diagnosis and follow-up of paracoccidioidomycosis: Comparison of counterimmunoelectrophoresis and complement fixation
dc.typeOtro


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