Article
Efficacy of an indirect immunofluorescence test in the diagnosis of canine leishmaniosis
Registro en:
FIGUEIREDO, F. B. et al. Efficacy of an indirect immunofluorescence test in the diagnosis of canine leishmaniosis. The Veterinary Journal, v.186, p.123-124, 2010.
1090-0233
10.1016/j.tvjl.2009.06.030
1532-2971
Autor
Figueiredo, F. B.
Madeira, M. F.
Menezes, R. C.
Pacheco, R. S.
Pires, M. Q.
Furtado, M. C.
Pinto, A. G.
Schubach, T. M. P.
Resumen
Of 146 dogs from a visceral leishmaniosis-endemic area that tested seronegative by indirect immunofluorescence (IIF) on blood samples collected on filter paper (IIFp), 51 (34.9%) and 10 (6.8%) tested positive by IIF on serum samples (IIFs) and enzyme immunoassay, respectively. Three samples (2.0%) tested positive by PCR. Leishmania chagasi was isolated from the skin of five (3.4%) dogs. Amastigote forms were identified in two of these five animals following histopathological and immunohistochemical examination. The findings highlight that detection methods such as IIFp can permit dogs infected with L. chagasi to remain undetected in endemic areas with attendant consequences for the epidemiology of infection both in the canine and human populations. 2030-01-01