Gene discovery in the tropical scallop Nodipecten nodosus: Construction and sequencing of a normalized cDNA library

dc.creatorAmerico, Juliana Alves
dc.creatorDondero, Francesco
dc.creatorMoraes, Milton Ozório
dc.creatorAllodi, Silvana
dc.creatorRebelo, Mauro de Freitas
dc.date2016-01-19T14:31:06Z
dc.date2016-01-19T14:31:06Z
dc.date2013
dc.date.accessioned2023-09-26T21:02:30Z
dc.date.available2023-09-26T21:02:30Z
dc.identifierAMERICO, Juliana Alves; et al. Marine Environmental Research, v.91, p.34-40, 2013.
dc.identifier0141-1136
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12556
dc.identifier10.1016/j.marenvres.2013.03.008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8868185
dc.descriptionWe report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0 106 cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectEST
dc.subjectmRNA
dc.subjectLion’s Paw scallop
dc.subjectAquaculture
dc.subjectStress
dc.subjectRNA Mensageiro
dc.subjectEstresse
dc.subjectBiblioteca Gênica
dc.titleGene discovery in the tropical scallop Nodipecten nodosus: Construction and sequencing of a normalized cDNA library
dc.typeArticle


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