Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants

dc.creatorFarias, Beatriz Oliveira
dc.creatorBianco, Kayo
dc.creatorNascimento, Ana Paula Alves
dc.creatorGonçalves de Brito, Andressa Silva
dc.creatorMoreira, Thais Costa
dc.creatorClementino, Maysa Mandetta
dc.date2022-03-24T12:04:40Z
dc.date2022-03-24T12:04:40Z
dc.date2022
dc.date.accessioned2023-09-26T21:02:29Z
dc.date.available2023-09-26T21:02:29Z
dc.identifierFARIAS, Beatriz Oliveira et al. Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants. Microbial Drug Resistance, v. 0, n. 0, p. 1-9, 2022.
dc.identifier1931-8448
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51850
dc.identifier10.1089/mdr.2021.0254
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8868180
dc.descriptionThe emergence of vancomycin-resistant Enterococcus faecium (Efm) harboring vanA gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses a genomic investigation of vanA-carrying Efm from WWTPs in Brazil. Samples from five WWTPs supplied with sewage from different sources were evaluated. Here we present whole-genome sequencing of eight vanA-Efm isolates performed on Illumina MiSeq platform. All these isolates presented multidrug-resistant profile, and five strains were from treated wastewater. Multiple antimicrobial resistance genes (ARGs) were found, such as aph(3′)-IIIa, ant(6′)-Ia, erm(B), and msrC, some of them being allocated in plasmids. The virulence profile was predominantly constituted by efaAfm and acm genes and all isolates, except for one, were predicted as human pathogens. Multilocus sequence typing analysis revealed a new allele and five different STs, three previously described (ST32, ST168, and ST253) and two novel ones (ST1893 and ST1894). Six strains belonged to CC17, often associated with hospital outbreaks. As far as our knowledge, no genomic studies of vanA-Efm recovered from WWTPs revealed isolates belonging to CC17 in Brazil. Therefore, our findings point to the environmental spread of Efm carrying multiple ARGs.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectVancomycin-resistant enterococci (VRE)
dc.subjectAntibiotic resistance
dc.subjectWhole-genome sequencing
dc.subjectWastewater
dc.subjectEnterococos Resistentes à Vancomicina
dc.subjectResistência Microbiana a Medicamentos
dc.subjectSequenciamento Completo do Genoma
dc.subjectÁguas Residuárias
dc.titleGenomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
dc.typeArticle


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