Phenotypic and genotypic features of new autoagglutinating Bacillus thuringiensis strains

dc.creatorChaves, J. Q.
dc.creatorCavados, C. F. G.
dc.creatorRabinovitch, L.
dc.date2019-04-16T17:47:10Z
dc.date2019-04-16T17:47:10Z
dc.date2008
dc.date.accessioned2023-09-26T21:00:53Z
dc.date.available2023-09-26T21:00:53Z
dc.identifierCHAVES, J. Q.; CAVADOS, C. F. G.; RABINOVICH, L. Phenotypic and genotypic features of new autoagglutinating Bacillus thuringiensis strains. Journal of Invertebrate Pathology, v. 98, p. 85-92, 2008.
dc.identifier0022-2011
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32535
dc.identifier10.1016/j.jip.2008.01.002
dc.identifier1096-0805
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8867741
dc.descriptionA total of 28 autoagglutinating strains of Bacillus thuringiensis were isolated from different ecologic niches and distinct sites. Twenty-six strains demonstrated toxicity to mosquito larvae of Aedes aegypti and Culex quinquefasciatus. The electrophoretic protein profiles of the crystal components were studied. Twenty-three out of the 28 strains showed the same larvicidal activity and the same protein profiles as B. thuringiensis serovar israelensis. Using isoenzyme analysis (MLEE), it was observed the presence of three electrophoretic types (ETs). The mosquitocidal strains grouped into one ET. The random amplified polymorphic DNA analysis (RAPD) was evaluated using six primers, which demonstrated three different patterns for the 28 autoagglutinating strains, allowing correlation of the profiles obtained with the toxicity observed in the bioassays. The RAPD patterns for mosquitocidal strains were identical to the one of serovar israelensis. However, to strains of low toxicity, each primer generated distinctive RAPD patterns, which demonstrated that these strains belong to different serovars. Although the antigenic classification the 26 autoagglutinating strains of B. thuringiensis could not be determined by classical flagellar serotyping, MLEE and RAPD profiles proved these strains to be compatible with B. thuringiensis serovar israelensis.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectBacillus thuringiensis
dc.subjectAedes aegypti
dc.subjectCulex quinquefasciatus
dc.subjectAedes aegypti
dc.subjectCepas de mosquitos
dc.subjectBacillus thuringiensis
dc.subjectAutoagglutinating strains
dc.subjectAedes aegypti
dc.subjectCulex quinquefasciatus
dc.subjectMosquitocidal strains
dc.subjectMLEE
dc.subjectSDS-PAGE
dc.subjectRAPR-PCR
dc.subjectEletroforese em Gel de Poliacrilamida
dc.subjectTécnica de Amplificação ao Acaso de DNA Polimórfico
dc.titlePhenotypic and genotypic features of new autoagglutinating Bacillus thuringiensis strains
dc.typeArticle


Este ítem pertenece a la siguiente institución