Article
HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
Registro en:
Freitas, Maria Audilene de et al. HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae). 3 Biotech, v. 12, n. 1, p. 1-16, 2022.
2190-5738
doi.org/10.1007/s13205-022-03126-1
Autor
Freitas, Maria Audilene de
Cruz, Rafael Pereira da
Santos, Antonia Thassya Lucas dos
Bezerra, José Weverton Almeida
Machado, Antonio Júdson Targino
Santos, Joycy Francely Sampaio dos
Rocha, Janaina Esmeraldo
Boligon, Aline Augusti
Bezerra, Camila Fonseca
Freitas, Thiago Sampaio de
Silva, Maria Karollyna do Nascimento
Mendonça, Ana Cleide Alcântara Morais
Costa, José Galberto Martins da
Coutinho, Henrique Douglas Melo
Cunha, Francisco Assis Bezerra da
Ribeiro Filho, Jaime
Braga, Maria Flaviana Bezerra Morais
Resumen
Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Federal de Santa Maria. Santa Maria, RS, Brasil. Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Fundação Cearense de Apoio ao Desenvolvimento (FUNCAP). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Financiadora de Estudos e Projetos (FINEP). Spondias mombin is used in the folk medicine for the treatment of diarrhea and dysentery, indicating that extracts obtained from this species may present pharmacological activities against pathogenic microorganisms. The purpose of this work was to investigate the chemical composition and evaluate the antimicrobial activity of extracts obtained from the leaves (aqueous) and bark (hydroethanolic) of S. mombin both as single treatments and in combination with conventional drugs. Following a qualitative chemical prospection, the extracts were analyzed by HPLC–DAD. The antimicrobial activities were evaluated by microdilution. The combined activity of drugs and extracts was verified by adding a subinhibitory concentration of the extract in the presence of variable drug concentrations. The Minimum Fungicidal Concentration (MFC) was determined by a subculture of the microdilution test, while the effect of the in vitro treatments on morphological transition was analyzed by subculture in moist chambers. While the qualitative analysis detected the presence of phenols and flavonoids, the HPLC analysis identified quercetin, caffeic acid, and catechin as major components in the leaf extract, whereas kaempferol and quercetin were found as major compounds in the bark extract. The extracts showed effective antibacterial activities only against the Gram-negative strains. With regard to the combined activity, the leaf extract potentiated the action of gentamicin and imipenem (against Staphylococcus aureus), while the bark extract potentiated the effect of norfloxacin (against S. aureus), imipenem (against Escherichia coli), and norfloxacin (against Pseudomonas aeruginosa). A more significant antifungal (fungistatic) effect was achieved with the bark extract (even though at high concentrations), which further enhanced the activity of fluconazole. The extracts also inhibited the emission of filaments by Candida albicans and Candida tropicalis. Together, these findings suggest that that the extract constituents may act by favoring the permeability of microbial cells to conventional drugs, as well as by affecting virulence mechanisms in Candida strains.