Article
DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
Registro en:
PINTO, Israel de Souza et al. DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil. PLoS One, vol. 10, n. 10, p. e0140636, 2015
1932-6203
10.1371/journal.pone.0140636. eCollection 2015.
Autor
Pinto, Israel de Souza
Chagas, Bruna Dias das
Rodrigues, Andressa Alencastre Fuzari
Ferreira, Adelson Luiz
Rezende, Helder Ricas
Bruno, Rafaela Vieira
Falqueto, Aloisio
Andrade-Filho, José Dilermando
Galati, Eunice Aparecida Bianchi
Shimabukuro, Paloma Helena Fernandes
Brazil, Reginaldo Peçanha
Peixoto, Alexandre Afranio
Resumen
DNA barcoding has been an effective tool for species identification in several animal groups. Here, we used DNA barcoding to discriminate between 47 morphologically distinct species of Brazilian sand flies. DNA barcodes correctly identified approximately 90% of the sampled taxa (42 morphologically distinct species) using clustering based on neighbor-joining distance, of which four species showed comparatively higher maximum values of divergence (range 4.23-19.04%), indicating cryptic diversity. The DNA barcodes also corroborated the resurrection of two species within the shannoni complex and provided an efficient tool to differentiate between morphologically indistinguishable females of closely related species. Taken together, our results validate the effectiveness of DNA barcoding for species identification and the discovery of cryptic diversity in sand flies from Brazil.