Article
Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
Registro en:
FERRAZ, r. p. c. et al. Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae). Flavour and Fragrance Journal, v. 29, n. 4, p.228-232, 2014.
10.1002/ffj.3199
Autor
Ferraz, Rosana Paula Cruz
Bomfim, Diogo S
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Pinheiro, Maria Lúcia Bélem
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
Resumen
Guatteria blepharophylla Mart. (synonym Guatteriopsis blepharophylla Mart.) and Guatteria hispida (R.E. Fr.)
Erkens & Maas (synonym Guatteriopsis hispida R.E. Fr.) belong to the Annonaceae family and are found in the Brazilian
and Colombian Amazon basin. Both species are popularly known as ‘envira’ or ‘envireira’. In the present study, the leaf
essential oils of G. blepharophylla (EOGB) and G. hispida (EOGH) were selected to investigate their cytotoxic effects. Tumour
cell lines were treated with increasing concentrations of both essential oils for 72 h and analysed by a methyl-[3H]thymidine
incorporation assay. The pro-apoptotic effect of these essential oils was assessed in HepG2 cells by morphological analysis
(using haematoxylin/eosin staining and acridine orange/ethidium bromide staining), flow cytometry (cell membrane integrity
and internucleosomal DNA fragmentation analysis) and a caspase-3 activation assay after 24 h incubation. Both essential oils
displayed potent cytotoxicity in different tumour cell lines. EOGB showed IC50 values from 6.03 to 16.46 ìg/ml for HepG2 and
K562 cell lines, and EOGH showed IC50 values from 5.45 to 24.89 ìg/ml for HepG2 and K562 cell lines, respectively. Cell
morphologies consistent with apoptosis and a remarkable activation of caspase-3 were observed in the HepG2 cells treated
with essential oils for 24 h. Significant increases in internucleosomal DNA fragmentation without altered membrane integrity
were also found. In conclusion, both essential oils investigated were able to inhibit tumour cell proliferation and induce cell
death by apoptosis pathways.