Article
Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features
Registro en:
SILVA, Helen Aguiar Lemes da; et al. Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features. Rev. Soc. Bras. Med. Trop., v.48, n.5, p. 560-567, Sept./Oct. 2015.
0037-8682
10.1590/0037-8682-0183-2015
1678-9849
Autor
Silva, Helen Aguiar Lemes da
Lima, Gabriel Silva de
Boité, Mariana Côrtes
Porrozzi, Renato
Hueb, Marcia
Damazo, Amilcar Sabino
Resumen
Introduction: The aim of this study was quantify annexin A1 expression in macrophages and cluster of differentiation 4 (CD4)+
and cluster of differentiation 8 (CD8)+ T cells from the skin of patients with cutaneous leishmaniasis (n=55) and correlate with
histopathological aspects. Methods: Infecting species were identifi ed by polymerase chain reaction-restriction fragment length
polymorphism, and expression of annexin A1 was analyzed by immunofl uorescence. Results: All patients (n = 55) were infected
with Leishmania braziliensis. Annexin A1 was expressed more abundantly in CD163+ macrophages in infected skin (p < 0.0001)
than in uninfected skin. In addition, macrophages in necrotic exudative reaction lesions expressed annexin A1 at higher levels
than those observed in granulomatous (p < 0.01) and cellular lesions p < 0.05). This difference might be due to the need to clear
both parasites and necrotic tissue from necrotic lesions. CD4+ cells in cellular lesions expressed annexin A1 more abundantly
than did those in necrotic (p < 0.05) and granulomatous lesions (p < 0.01). Expression in CD8+ T cells followed the same
trend. These differences might be due to the pervasiveness of lymphohistiocytic and plasmacytic infi ltrate in cellular lesions.
Conclusions: Annexin A1 is differentially expressed in CD163+ macrophages and T cells depending on the histopathological
features of Leishmania-infected skin, which might affect cell activation.