A Method for Purifying Native Transthyretin from Human Plasma

dc.creatorSantana, Aline G.
dc.creatorCarvalho, Paulo Costa
dc.creatorZanchin, Nilson Ivo Tonin
dc.creatorSouza, Tatiana de Arruda Campos Brasil de
dc.date2016-08-26T12:46:28Z
dc.date2016-08-26T12:46:28Z
dc.date2015
dc.date.accessioned2023-09-26T20:54:36Z
dc.date.available2023-09-26T20:54:36Z
dc.identifierSANTANA, Aline G. et al. A Method for Purifying Native Transthyretin from Human Plasma. Biochem Pharmacol, Los Angeles, v. 4, n. 5, p. 1-5, 2015.
dc.identifier2167-0501
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15431
dc.identifier10.4172/2167-0501.1000187
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866013
dc.descriptionTransthyretin is a homotetrameric thyroid-hormone-transporting protein that binds to the retinol binding protein thus being involved in metabolism, growth, fertility, homeostasis of the cardiovascular and central nervous system, cell differentiation, reproduction, development and maintenance the cognitive processes during aging. Currently, there are several methodologies for natively purifying TTR from plasma, serum, tears, and amyloid fibrils; however, these procedures are laborious. Herein, a low-cost and simple protocol to purify TTR from human plasma is described. It involves the separation of plasma proteins by size exclusion and DEAE chromatography. The homogeneity was assessed by SDS-PAGE and by tandem mass spectrometry using an Orbitrap-XL (Thermo, San Jose-CA).
dc.formatapplication/pdf
dc.languagepor
dc.rightsopen access
dc.subjectTransthyretin
dc.subjectPlasma isolation
dc.titleA Method for Purifying Native Transthyretin from Human Plasma
dc.typeArticle


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