Article
Identification of phenotypic markers of B cells from patients with Chagas disease.
Registro en:
GUSMÃO, Rafaelle Christine Gomes Fares et al. Identification of phenotypic markers of B cells from patients with Chagas disease. Parasite Immunol., v. 35, n. 7-8, p. 214-23, 2013
0141-9838
10.1111/pim.12038
Autor
Gusmão, Rafaelle Christine Gomes Fares
Oliveira, Rodrigo Correa de
Araujo, Fernanda Fortes De
Keesen, Tatjana Souza Lima
Chaves, Ana Thereza
Fiuza, Jacqueline Araújo
Ferreira, Karine Silvestre
Rocha, Manoel Otávio da Costa
Gomes, Juliana Assis Silva
Resumen
Chagas disease was discovered more than a hundred years ago, but its pathogenesis is still not completely understood. Autoimmunity is one of the mechanisms shown to contribute to its pathogenesis, which may indicate an important participation of B lymphocytes. Patients with Chagas disease have shown increased percentage of B cells producing IL-10. However, there are no reports of the phenotypic markers of B cells producing IL-10 in patients with Chagas disease. For the first time in the literature, we evaluated the phenotypic profile of distinct markers of B cells from peripheral blood of noninfected individuals and patients with Chagas disease. Our results showed that patients with Chagas disease had a higher expression of CD21 and CD24 on the surface of CD19+ B cells, while CD43 and CD23 were expressed equally in all groups. Moreover, the expression of MHC-II (HLA-DR), CD80, CD86, caspase-3, granzyme B and intracellular IL-10 and TGF-β by CD19+ B cells was higher in patients with Chagas disease. The results of IL-10 production within CD19+ CD5+ CD1d+ B cells showed a higher percentage of this cytokine in patients with Chagas disease. Thus, our data bring a new knowledge about distinct markers of B cells in immune responses of Chagas disease. 2100-01-01