Article
Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells
Registro en:
PIRES, Weverson Luciano et al. Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells. Toxicology in Vitro, v. 41, p. 30-41, 2017.
0887-2333
10.1016/j.tiv.2017.02.003
1879-3177
Autor
Pires, Weverson Luciano
Castro, Onassis Boeri de
Kayano, Anderson Makoto
Setúbal, Sulamita da Silva
Pontes, Adriana Silva
Nery, Neriane Monteiro
Paloschi, Mauro Valentino
Pereira, Soraya dos Santos
Stábeli, Rodrigo Guerino
Fernandes, Carla Freire Celedônio
Soares, Andreimar Martins
Zuliani, Juliana Pavan
Resumen
BjcuL is a C-type lectin with specificity for the binding of β-D-galactose units isolated from Bothrops jararacussu
venom. It triggers cellular infiltration in post capillary venules, increases edema and vascular permeability inmurine
models, contributes to in vitro neutrophil activation and modulates macrophage functional activation towards
an M1 state. The purpose of this study was to investigate the effect of BjcuL on human peripheral blood
mononuclear cells (PBMCs) activation with a focus on PBMCs proliferation and inflammatory mediators release.
Results showed that BjcuL is not toxic to PBMCs, that BjcuL inhibits PBMCs proliferation and that it stimulates
PBMCs to produce superoxide anion and hydrogen peroxide, primarily via lymphocyte stimulation, but does
not stimulate the production of nitric oxide and PGE2. These results demonstrate that BjcuL has an immunomodulatory
effect on PBMCs. Further studies are needed to confirm the immunomodulatory effect of BjcuL, to elucidate
the molecular mechanisms of action responsible for its effects and to determine its potential application as
an immunopharmacological and biotechnological tool.