Article
Whole cells in enantioselective reduction of benzyl acetoacetate
Registro en:
RIBEIRO, Joyce Benzaquem et al. Whole cells in enantioselective reduction of benzyl acetoacetate. Brazilian Journal of Microbiology, v. 45, n. 3, p. 929-932, Sept. 2014.
1678-4405
10.1590/S1517-83822014000300023
Autor
Ribeiro, Joyce Benzaquem
Ramos, Aline de Souza
Lopes, Raquel de Oliveira Silva
Silva, Gabriela Veloso Vieira da
Souza, Rodrigo Octavio Mendonça Alves de
Resumen
The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.