Article
A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
Registro en:
COSTA, Eliane Veiga da; et al. A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures. Mem Inst Oswaldo Cruz, v.107, n.5, p.698-701, Aug. 2012.
1678-8080
10.1590/S0074-02762012000500022
Autor
Costa, Eliane Veiga da
Campos, Renata de Mendonça
Tavares, Fernando Neto
Grégio, Cátia Regina Valério
Burlandy, Fernanda Marcicano
Silva, Edson Elias da
Resumen
Outbreaks caused by vaccine-derived polioviruses are challenging the final eradication of paralytic poliomyelitis.
Therefore, the surveillance of the acute flaccid paralysis cases based on poliovirus isolation and characterization
remains an essential activity. Due to the use of trivalent oral poliovirus vaccine (OPV), mixtures containing
more than one serotype of Sabin-related polioviruses are frequently isolated from clinical samples. Because each
poliovirus isolate needs to be individually analyzed, we designed polymerase chain reaction primers that can selectively
distinguish and amplify a genomic segment of the three Sabin-related poliovirus serotypes present in mixtures,
thus, optimizing the diagnosis and providing prompt information to support epidemiologic actions.