Article
qPCR-High resolution melt analysis for drug susceptibility testing of Mycobacterium leprae directly from clinical specimens of leprosy patients
Registro en:
ARAUJO, Sergio; et al. qPCR-High resolution melt analysis for drug susceptibility testing of Mycobacterium leprae directly from clinical specimens of leprosy patients. PLoS Negl Trop Dis., v.11, n.6, e0005506, 18p, June 2017.
1935-2727
10.1371/journal.pntd.0005506
Autor
Araujo, Sergio
Goulart, Luiz Ricardo
Truman, Richard W.
Goulart, Isabela Maria B.
Vissa, Varalakshmi
Li, Wei
Matsuoka, Masanori
Suffys, Philip
Fontes, Amanda B.
Rosa, Patricia S.
Scollard, David M.
Williams, Diana L.
Resumen
Real-Time PCR-High Resolution Melting (qPCR-HRM) analysis has been recently described for rapid drug susceptibility testing (DST) of Mycobacterium leprae. The purpose of the current study was to further evaluate the validity, reliability, and accuracy of this assay for M. leprae DST in clinical specimens.