Article
The Main Molecular and Serological Methods for Diagnosing COVID-19: An Overview Based on the Literature
Registro en:
MACHADO, Bruna Aparecida Souza et al. The Main Molecular and Serological Methods for Diagnosing COVID-19: An Overview Based on the Literature. Viruses, v. 13, p. 1-36, 2021.
1999-4915
10.3390/v13010040
Autor
Machado, Bruna Aparecida Souza
Hodel, Katharine Valéria Saraiva
Barbosa Júnior, Valdir Gomes
Soares, Milena Botelho Pereira
Badaró, Roberto
Resumen
Diagnostic tests have been considered as the main alternative for the control of coronavirus
disease (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),
as a correct diagnosis allows for decision making when facing the disease, particularly as there is
a lack of effective therapeutic protocols and vaccines. Thus, in this review, we summarized the
main diagnostic approaches currently available for the diagnosis of SARS-CoV-2 infection in humans
based on studies available in article databases. The tests can be organized into two main categories:
nucleic acid-based tests, recommended for the initial detection of the virus, and serological tests,
recommended for assessing the disease progression. The studies have shown that the performance of
diagnostic methods depends on different factors, such as the type of samples and the characteristics of
each assay. It was identified that the positivity of the tests is mainly related to the onset of symptoms.
We also observed that point-of-care diagnoses are considered as one of the main trends in this area,
due to the low-cost and simplicity of the assay; however, the analytical performance must be critically
analyzed. Thus, the COVID-19 pandemic has highlighted the critical role of diagnostic technologies
in the control of infectious diseases.