Article
Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
Registro en:
CERQUEIRA, G. M. et al. Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates. Journal of Medical Microbiology, v. 58, n. 9, p. 1173–1181, 2009.
0022-2615
10.1099/jmm.0.009175-0
Autor
Cerqueira, Gustavo Maia de
McBride, Alan John Alexander
Picardeau, Mathieu
Ribeiro, Samuel G
Moreira, Ângela N
Morel, Viviane
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Dellagostin, Odir Antonio
Resumen
The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study
used PCR to demonstrate the presence of lig genes among serovars from a collection of
leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited
number of pathogenic serovars, the ligB gene was distributed ubiquitously among all pathogenic
strains. None of the lig genes were detected among intermediate or saprophytic Leptospira
species. It was also shown that, similar to the previously characterized secY gene, a short specific
PCR fragment of ligB could be used to correctly identify pathogenic Leptospira species. These
findings demonstrate that ligB is widely present among pathogenic strains and may be useful for
their reliable identification and classification.
Materias
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