Leishmania amazonensis: participation of regulatory T and B cells in the in vitro priming (PIV) of CBA/J spleen cells susceptible response.

dc.creatorVeras, Patrícia Sampaio Tavares
dc.creatorBorges, Marcus Welby
dc.creatorSantana, Cláudia Dias de
dc.creatorNihei, Jorge Sadao
dc.creatorCardillo, Fabíola
dc.creatorFreitas, Luiz Antonio Rodrigues de
dc.date2014-06-02T17:38:10Z
dc.date2014-06-02T17:38:10Z
dc.date2006
dc.date.accessioned2023-09-26T20:38:11Z
dc.date.available2023-09-26T20:38:11Z
dc.identifierVERAS, P. S. T. et al. Leishmania amazonensis: participation of regulatory T and B cells in the in vitro priming (PIV) of CBA/J spleen cells susceptible response. Experimental Parasitology, v. 113, n. 3, p. 201-205, 2006.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7750
dc.identifier10.1016/j.exppara.2006.01.008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861047
dc.descriptionCBA/J mice are resistant to Leishmania major and susceptible to Leishmania amazonensis. Early events determine infection outcome. Until now, PIV (in vitro priming) immune response to L. amazonensis has not been assessed. Herein, we have shown that compared to L. major, L. amazonensis induced higher parasite burden associated to similar IL-4, IFN- , and TNF- mRNA expressions and IFN- and IL-10 levels. Although similar amounts of IL-10 were detected, the frequency of intracellular IL-10 positive B cells was enhanced in spleen cells stimulated with anti-CD3/anti-CD28, or anti-CD3/anti-CD28 and L. amazonensis, compared to L. major-stimulation. Interestingly, IL-10- producing B cells were reduced in response to anti-CD3/anti-CD28 stimulation combined with L. major compared to the other groups. L. amazonensis may favor T regulatory cell development, since 40% of all the CD4+CD25+ were CD25high cells. These data suggest that in PIV, susceptibility to L. amazonensis is not related to Th cell polarization, but to the presence and activity of regulatory T and B cells.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectCBA/J mice
dc.subjectPIV
dc.subjectLeishmania amazonensis
dc.subjectLeishmania major
dc.subjectRegulatory T and B cells
dc.subjectLinfócitos B/imunologia
dc.subjectLeishmania mexicana/imunologia
dc.subjectLeishmaniose Cutânea/imunologia
dc.subjectBaço/imunologia
dc.subjectLinfócitos T/imunologia
dc.subjectAnálise de Variância
dc.subjectAnimais
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectExpressão Gênica
dc.subjectInterações Hospedeiro-Parasita/imunologia
dc.subjectHipoxantina Fosforribosiltransferase/biossíntese
dc.subjectImunidade Celular
dc.subjectInterferon gama/biossíntese
dc.subjectInterleucina-10/biossíntese
dc.subjectCamundongos Endogâmicos CBA
dc.subjectCamundongos
dc.subjectÓxido Nítrico/biossíntese
dc.subjectRNA Mensageiro/biossíntese
dc.subjectReação em Cadeia da Polimerase Via Transcriptase Reversa
dc.subjectBaço/citologia
dc.subjectFator de Necrose Tumoral alfa/biossíntese
dc.titleLeishmania amazonensis: participation of regulatory T and B cells in the in vitro priming (PIV) of CBA/J spleen cells susceptible response.
dc.typeArticle


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