Article
Leishmania amazonensis: participation of regulatory T and B cells in the in vitro priming (PIV) of CBA/J spleen cells susceptible response.
Registro en:
VERAS, P. S. T. et al. Leishmania amazonensis: participation of regulatory T and B cells in the in vitro priming (PIV) of CBA/J spleen cells susceptible response. Experimental Parasitology, v. 113, n. 3, p. 201-205, 2006.
0014-4894
10.1016/j.exppara.2006.01.008
Autor
Veras, Patrícia Sampaio Tavares
Borges, Marcus Welby
Santana, Cláudia Dias de
Nihei, Jorge Sadao
Cardillo, Fabíola
Freitas, Luiz Antonio Rodrigues de
Resumen
CBA/J mice are resistant to Leishmania major and susceptible to Leishmania amazonensis. Early events determine infection outcome. Until
now, PIV (in vitro priming) immune response to L. amazonensis has not been assessed. Herein, we have shown that compared to L. major, L.
amazonensis induced higher parasite burden associated to similar IL-4, IFN- , and TNF- mRNA expressions and IFN- and IL-10 levels.
Although similar amounts of IL-10 were detected, the frequency of intracellular IL-10 positive B cells was enhanced in spleen cells stimulated
with anti-CD3/anti-CD28, or anti-CD3/anti-CD28 and L. amazonensis, compared to L. major-stimulation. Interestingly, IL-10- producing B
cells were reduced in response to anti-CD3/anti-CD28 stimulation combined with L. major compared to the other groups. L. amazonensis
may favor T regulatory cell development, since 40% of all the CD4+CD25+ were CD25high cells. These data suggest that in PIV, susceptibility
to L. amazonensis is not related to Th cell polarization, but to the presence and activity of regulatory T and B cells.
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