Article
A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
Registro en:
SILVA, M. A. L. da et al. A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 2, p. 193–197, abr. 2014.
1678-9849
10.1590/0037-8682-0233-2013
Autor
Silva, Maria Almerice Lopes da
Medeiros, Zulma
Soares, Cynthia Regina Pedrosa
Silva, Elis Dionísio da
Miranda-Filho, Demócrito Barros
Melo, Fábio Lopes de
Resumen
Polymerase chain reaction (PCR) may offer an alternative diagnostic option when clinical signs and symptoms suggest visceral leishmaniasis (VL) but microscopic scanning and serological tests provide negative results. PCR using urine is sensitive enough to diagnose human visceral leishmaniasis (VL). However, DNA quality is a crucial factor for successful amplification.
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