Article
A new repetitive DNA sequence from Trypanosoma cruzi
Registro en:
LIMA, Leila de Mendonça; TRAUB-CSEKO, Yara M. A new repetitive DNA sequence from Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 4, p. 475, Oct./Dec. 1991.
0074-0276
10.1590/S0074-02761991000400020
1678-8060
Autor
Lima, Leila de Mendonça
Traub-Cseko, Yara M.
Resumen
Tandemly repeated DNA sequences are found in the genome of higher eukaryotes, and have also been demonstrated in Trypanosoma cruzi. Repeated DNA sequences are potentially useful for the diagnostic detection of T. cruzi (A. Gonzales et al., 1984, Proc. Natl. Acad. Sci. USA, 81: 3356-3360). We have isoleted two clones from a genomic library of T. cruzi (Y strain) that contain, in one clone a family of at least seven copies of a repetitive sequence of approximately 600 base pairs, and in the other an independent copy of the same sequence. One copy of the repetition (HSP) and the independent clone (HCR) were sequenced by the Sanger procedure (Fig.). This sequence hybridized to four strains of T. cruzi tested and did not hybridize to eleven species of trypanosotids from five different Genera, being a good candidate for diagnostic assays.