Internal Transcribed Spacer rRNA Gene-Based Phylogenetic Reconstruction Using Algorithms with Local and Global Sequence Alignment for Black Yeasts and Their Relatives

dc.creatorCaligiorne, Rachel Basques
dc.creatorLicinio, P.
dc.creatorDupont, J.
dc.creatorHoog, G. S. de
dc.date2013-08-05T12:54:58Z
dc.date2013-08-05T12:54:58Z
dc.date2005
dc.date.accessioned2023-09-26T20:35:37Z
dc.date.available2023-09-26T20:35:37Z
dc.identifierCALIGIORNE, Rachel Basques et al. (2005). Internal transcribed spacer rRNA gene-based phylogenetic reconstruction using algorithms with local and global sequence alignment for black yeasts and their relatives. J. Clin. Microbiol., v. 43, n. 6, p. 2816-2823, June 2005.
dc.identifier0095-1137
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/6741
dc.identifier10.1128/JCM.43.6.2816-2823.2005
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860214
dc.descriptionSequences of rRNA gene Internal transcribed spacer (ITS) of a standard set of black yeast-like fungal pathogens were compared using two methods: local and global alignments. The latter is based on DNA-walk divergence analysis. This method has become recently available as an algorithm (DNAWD program) which converts sequences into three-dimensional walks. The walks are compared with, or fit to, each other generating global alignments. The DNA-walk geometry defines a proper metric used to create a distance matrix appropriated for phylogenetic reconstruction. In this work, the analyses were carried out for species currently classified in Capronia, Cladophialophora, Exophiala, Fonsecaea, Phialophora, and Ramichloridium. Main groups were verified by small-subunit rRNA gene data. DNAWD applied to ITS2 alone enabled species recognition as well as phylogenetic reconstruction reflecting clades discriminated in small-subunit rRNA gene phylogeny, which was not possible with any other algorithm using local alignment for the same data set. It is concluded that DNAWD provides rapid insight into broader relationships between groups using genes that otherwise would be hardly usable for this purpose
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectSequences of rRNA
dc.subjectDNAWD program
dc.subjectPhylogenetic reconstruction
dc.titleInternal Transcribed Spacer rRNA Gene-Based Phylogenetic Reconstruction Using Algorithms with Local and Global Sequence Alignment for Black Yeasts and Their Relatives
dc.typeArticle


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