Article
Probiotic treatment during neonatal age provides optimal protection against experimental asthma through the modulation of microbiota and T cells
Registro en:
NUNES, Carolina Fraga; et al. Probiotic treatment during neonatal age provides optimal protection against experimental asthma through the modulation of microbiota and T cells. International Immunology, v. 30, n.4, p.155–169, 2018.
0953-8178
10.1093/intimm/dxy011
1460-2377
Autor
Nunes, Caroline Fraga
Nogueira, Jeane S.
Vianna, Pedro Henrique Oliveira
Ciambarella, Bianca Torres
Rodrigues, Patrícia Machado
Miranda, Karla Rodrigues
Lobo, Leandro Araújo
Domingues, Regina Maria Cavalcanti Pillotto
Busch, Mileane
Atella, Georgia Correa
Vale, André Macedo
Bellio, Maria
Nóbrega, Alberto
Canto, Fábio B.
Fucs, Rita
Resumen
The incidence of allergic diseases, which increased to epidemic proportions in developed countries over the last few decades, has been correlated with altered gut microbiota colonization. Although probiotics may play a critical role in the restoration of gut homeostasis, their efficiency in the control of allergy is controversial. Here, we aimed to investigate the effects of probiotic treatment initiated at neonatal or adult ages on the suppression of experimental ovalbumin (OVA)-induced asthma. Neonatal or adult mice were orally treated with probiotic bacteria and subjected to OVA-induced allergy. Asthma-like symptoms, microbiota composition and frequencies of the total CD4+ T lymphocytes and CD4+Foxp3+ regulatory T (Treg) cells were evaluated in both groups. Probiotic administration to neonates, but not to adults, was necessary and sufficient for the absolute prevention of experimental allergen-induced sensitization. The neonatally acquired tolerance, transferrable to probiotic-untreated adult recipients by splenic cells from tolerant donors, was associated with modulation of gut bacterial composition, augmented levels of cecum butyrate and selective accumulation of Treg cells in the airways. Our findings reveal that a cross-talk between a healthy microbiota and qualitative features inherent to neonatal T cells, especially in the Treg cell subset, might support the beneficial effect of perinatal exposure to probiotic bacteria on the development of long-term tolerance to allergens. 2030-01-01