Preprint
Complete genome sequence of human T-cell lymphotropic type 1 from patients with different clinical profiles, including infective dermatitis
Registro en:
ARAÚJO, Thessika Hialla Almeida et al. Complete genome sequence of human T-cell lymphotropic type 1 from patients with different clinical profiles, including infective dermatitis. Infection, Genetics and Evolution, p. 1-29, 2020.
1567-1348
10.1016/j.meegid.2019.104166
Autor
Araújo, Thessika Hialla Almeida
Barreto, Fernanda Khouri
Menezes, Aline Dórea Luz
Lima, Clayton Pereira Silva de
Oliveira, Rodrigo Santos de
Lemos, Poliana da Silva
Castro Filho, Bernardo Galvão
Kashima, Simone
Farre, Lourdes
Bittencourt, Achilea Candida Lisboa
Carvalho, Edgar Marcelino de
Santos, Luciane Amorim
Rego, Filipe Ferreira de Almeida
Miranda, Aline Cristina Andrade Mota
Nunes, Márcio Roberto Teixeira
Alcântara, Luiz Carlos Júnior
Resumen
FAPESB and CNPq financial support. The HTLV-1 is the first human retrovirus and is associated with several clinical syndromes, however, the pathogenesis of these clinical manifestations is still not fully understood. Furthermore, there are few complete genomes publicly available, about 0.12 complete genomes per 10,000 infected individuals and the databases have a major deficiency of sequences information. This study generated and characterized 31 HTLV-1 complete genomes sequences derived from individuals with Tropical Spastic Paraparesis/HTLV-1-Associated Myelopathy (TSP/HAM), Adult T-cell leukemia/lymphoma (ATL), infective dermatitis associated to HTLV-1 (IDH) and asymptomatic patients. These sequences are associated to clinical and epidemiological information about the patients. The sequencing data generated on Ion Torrent PGM platform were assembled and mapped against the reference HTLV-1 genome. These sequences were genotyped as Cosmopolitan subtype, Transcontinental subgroup. We identified the variants in the coding regions of the genome of the different clinical profiles, however, no statistical relation was detected. This study contributed to increase of HTLV-1 complete genomes in the world. Furthermore, to better investigate the contribution of HTLV-1 mutations for the disease outcome it is necessary to evaluate the interaction of the viral genome and characteristics of the human host.