Article
In vitro leishmanicidal, antibacterial and antitumour potential of anhydrocochlioquinone A obtained from the fungus Cochliobolus sp
Registro en:
CAMPOS, Fernanda Fraga et al. In vitro leishmanicidal, antibacterial and antitumour potential of anhydrocochlioquinone A obtained from the fungus Cochliobolus sp. J Biosci Vol. 42, No. 4, p. 657–664, 2017
0250-5991
10.1007/s12038-017-9718-1
Autor
Campos, Fernanda Fraga
Ramos, Jonas Pereira
Oliveira, Djalma Menezes de
Alves, Tania Maria de Almeida
Fagundes, Elaine Maria de Souza
Zani, Carlos Leomar
Sampaio, Fabio Coelho
Converti, Attilio
Cota, Betania Barros
Resumen
The bioassay-guided fractionation of the ethyl acetate extract of the fungus Cochliobolus sp. highlighted leishmanicidal activity and allowed for anhydrocochlioquinone A (ANDC-A) isolation. MS, 1D and 2D NMR spectra of this compound were in agreement with those published in the literature. ANDC-A exhibited leishmanicidal activity with EC50 value of 22.4 lg/mL (44 lM) and, when submitted to the microdilution assay against Gram-positive and Gram-negative bacteria, showed a minimal inhibitory concentration against Staphylococcus aureus ATCC 25295 of 128 lg/mL (248.7 lM). It was also active against five human cancer cell lines, showing IC50 values from 5.4 to 20.3 lM. ANDC-A demonstrated a differential selectivity for HL-60 (SI 5.5) and THP-1 (SI 4.3) cell lines in comparison with Vero cells and was more selective than cisplatin and doxorubicin against MCF-7 cell line in comparison with human peripheral blood mononuclear cells. ANDC-A was able to eradicate clonogenic tumour cells at concentrations of 20 and 50 lM and induced apoptosis in all tumour cell lines at 20 lM. These results suggest that ANDC-A might be used as a biochemical tool in the study of tumour cells biochemistry as well as an anticancer agent with durable effects on tumours. 2025-01-01