Fungal cells are efficient in releasing to the extracellular space molecules that lack typical secretion signals, including cytoplasmic components. Studies developed during the last five years indicate that extracellular vesicle formation is involved in the traffic of these intracellular components to the extracellular space. The cellular origin of these vesicles, however, is still unknown. Here we review the potential mechanisms involved in formation of fungal extracellular vesicles and consequent release of fungal molecules to the outer cellular space. We also propose that these compartments can originate from cytoplasmic subtractions whose formation is dependent on plasma membrane reshaping.2024-01-01