Article
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs
Registro en:
VELOSO, Laura Barral et al. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International, v. 2020, Article ID 2615787, 8p, 2020.
2314-6133
2314-6141
Autor
Veloso, Laura Barral
Cardoso, Flávia de Oliveira
Charret, Karen dos Santos
Silva, Matheus Pereira de Sá
Côrtes, Luzia Monteiro de Castro
Calabrese, Kátia da Silva
Silva, Franklin Souza da
Sousa, Joel Fontes de
Rocha, Marília Fonseca
Alves, Carlos Roberto
Resumen
Leishmania spp. proteases have been proposed as virulence factors contributing to adaptive success these parasites to the
mammalian hosts. Since these enzymes are poorly studied in naturally infected dogs, this work aims to show the differences in
metalloprotease and cysteine proteases gene expression in ear edge skin of dogs naturally infected by Leishmania (Leishmania)
infantum. A cohort of dogs (n = 20) naturally infected by L. (L.) infantum was clinically classified as asymptomatic,
oligosymptomatic, and polysymptomatic and the parasite load range estimated. The analysis of proteases expression by RT-PCR
in the ear edge skin was also assessed, suggesting more transcripts of proteases in cDNA samples from polysymptomatic dogs
than oligosymptomatic and asymptomatic ones. Metalloprotease RT-PCR assays yielded products (202 bp) in all assessed cDNA
dog samples. In contrast, cysteine proteases transcripts (227 bp) had shown to be better detected in cDNA samples of
polysymptomatic dogs, compared with cDNA samples from asymptomatic and oligosymptomatic dogs. Predictive in silico
assays suggested that secondary structures of metalloproteasee mRNAs can be more stable than cysteine proteases at the skin
temperature of dogs. Evidence is presented that during natural infection of dogs by L. (L.) infantum, this parasite produces
transcripts of metalloprotease and cysteine protease RNA in the skin from asymptomatic, oligosymptomatic, and
polysymptomatic dogs.
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