Article
Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory.
Registro en:
CAMPOS, R.M.; ANDRADE, S.G. Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory. Memórias do Instituto Oswaldo Cruz, v. 91, n. 6, p. 795-800, nov.-dec. 1996.
0074-0276
Autor
Campos, Rozalia Mendes Figueira
Andrade, Sonia Gumes
Resumen
Several studies have shown a clonal structure of Trypanosoma cruzi and its possible correlation with the behavioral heterogeneity of the parasite strains. In the present study, the 21 SF strain, that have been maintained in laboratory by successive passages in mice, for more than 15 years, showing a stability of biological and isoenzymic characteristics has been cloned, with the objective of establishing the characters of its clones and subclones. With the technique of isolation of a single parasite from the blood of infected mice, 5 clones and 14 subclones have been obtained. After four passages into mice, inoculum of 10(5) was obtained for each clone and subclone and inoculated into mice weighing 10 to 12 g. These were used for the study of the biological behavior of the clones: evolution of parasitemia, morphology of blood forms and host mortality. For isoenzymic characterization, the clones and subclones were analyzed for ALAT, ASAT, GPI and PGM enzymes. Results have shown that the 5 clones and the 14 subclones disclosed a biological behavior similar to the parental strain, with minor variability of the parasitemic profiles and also the same isoenzymic patterns. These results confirm the stability of the 21 SF strain and indicate a clonal homogeneity of its populations. This is compatible with the hypothesis that the T. cruzi strains represent an equilibrium of either homogenous or heterogeneous populations.
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Putative Role of the Aldo-Keto Reductase from Trypanosoma cruzi in Benznidazole Metabolism
Garavaglia, Patricia Andrea; Garavaglia, Patricia Andrea; Garavaglia, Patricia Andrea; Garavaglia, Patricia Andrea; Garavaglia, Patricia Andrea; Laverriere, Marc; Laverriere, Marc; Laverriere, Marc; Laverriere, Marc; Laverriere, Marc; Cannata, Joaquin Juan Bautista; Cannata, Joaquin Juan Bautista; Cannata, Joaquin Juan Bautista; Cannata, Joaquin Juan Bautista; Cannata, Joaquin Juan Bautista; Garcia, Gabriela Andrea; Garcia, Gabriela Andrea; Garcia, Gabriela Andrea; Garcia, Gabriela Andrea; Garcia, Gabriela Andrea (American Society for MicrobiologyAmerican Society for MicrobiologyAmerican Society for MicrobiologyAmerican Society for MicrobiologyAmerican Society for Microbiology, 2016-05)Benznidazole (Bz), the drug used for treatment of Chagas' disease (caused by the protozoan Trypanosoma cruzi), is activated by a parasitic NADH-dependent type I nitroreductase (NTR I). However, several studies have shown ... -
Detección de Trypanosoma cruzi en tejido y sangre murina por PCR convencional y en tiempo real
Davies, Carolina; Poma, Hugo Ramiro; Cardozo, Rubén Marino; Mora, Maria Celia; Ramos, Federico; Rajal, Verónica Beatriz; Basombrio, Miguel Angel Manuel (Federación Bioquímica de la Provincia Buenos Aires, 2014-12)El objetivo del presente trabajo fue comparar la detección de ADN de Trypanosoma cruzi mediante PCR en tiempo real (qPCR) y PCR convencional en sangre periférica (n=25) y músculo esquelético (n=20) de ratones tratados ... -
Use of the polymerase chain reaction (PCR) for the therapeutic control of chronic Trypanosoma cruzi infection
Lacunza, Carlos Diego; Negrette, Olga Sánchez; Mora, María Celia; Bustos, María Fernanda García; Basombrío, Miguel Ángel