Article
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity
Registro en:
AGUIAR, P. H. P. et al. A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity. Veterinary Journal, v.173, n.1, p.158-166. jan., 2007.
1090-0233
10.1016/j.tvjl.2005.08.015
Autor
Aguiar, Paulo Henrique Palis
Melo, Stella Maria Barrouin
Franke, Carlos Roberto
Santos, Roberto Robson Borges dos
Silva, Tânia Maria Correia
Mengele Junior, José Orivaldo
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
Resumen
This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine
leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear
cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins
with apparent molecular weights of 180, 190, 205 and 220 kDa. The AB6 mAb recognised the majority of canine peripheral
blood leukocytes as determined by flow cytometry (97%). It also exhibited a broad reactivity pattern against lymphoid and
myeloid cells, inhibited the proliferation of mitogen-stimulated canine PBMC and did not recognise human PBMC and murine
splenocytes. The biochemical properties, cell and tissue specificity, and in vitro biological activity of the AB6 mAb indicate that
it recognises a canine CD45 homologue. The mAb could become a valuable diagnostic and research tool for the evaluation of
immune functions in dogs.